Dihydroxyacetone Kinase Activity in Dunaliella parva
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چکیده
منابع مشابه
The Plasma Membrane ATPase of Dunaliella parva
Plasma membrane Mg2+, Ca2+ ATPases were isolated from Dunaliella parva by differential centrifugation and subsequent sucrose gradient centrifugation and analyzed for their properties with special emphasis on ecophysiological requirements of this extremely salt-tolerant alga. Most properties (Vmaxand AfM-values, substrate specificity, vanadate and DES sensitivity, resistance against ouabain) ind...
متن کاملTwo isozymes of dihydroxyacetone phosphate reductase in dunaliella.
Two isoforms of dihydroxyacetone phosphate reductase were present in Dunaliella tertiolecta. The major form was located in the chloroplast and the minor form in the cytosol. The chloroplastic reductase eluted first from a DEAE cellulose column followed immediately by the cytosolic form. Both forms were unstable and cold labile. Addition of 5 millimolar dithiothreitol helped to stabilize the enz...
متن کاملIsolation, Characterization, and Partial Purification of a Reduced Nicotinamide Adenine Dinucleotide Phosphate-dependent Dihydroxyacetone Reductase from the Halophilic Alga Dunaliella parva.
An NADP(+)-dependent dihydroxyacetone reductase, which catalyzes specifically the reduction of dihydroxyacetone to glycerol, has been isolated from the halophilic alga Dunaliella parva. The enzyme has been purified about 220-fold. It has a molecular weight of about 65,000 and is highly specific for NADPH. The pH optima for dihydroxyacetone reduction and for glycerol oxidation are 7.5 and 9.2, r...
متن کاملGlycerol kinase and dihydroxyacetone kinase in rat brain.
The enzymatic phosphorylation of glycerol and dihydroxyacetone by ATP to ,sn-glycerol-3phosphate and dihydroxyacetone phosphate respectively in various subcellular fractions of rat hrain was studied. A sensitive radiochemical assay was used where the labelled phosphorylated products were separated from the radioactive substrates by high voltage paper electrophoresis and the radioactivity in the...
متن کاملDEAE-Dextran Induced Increase of Membrane Permeability and Inhibition of Photosynthesis in Dunaliella parva
DEAE-Dextran, Dunaliella, Membrane Permeability, Photosynthesis, Polycations As a prerequisite for studies o f the intracellular distribution o f enzymes o f the glycerol cycle in Dunaliella parva, the effect o f the polycation DEAE-dextran on the permeability o f membranes for various endogenous compounds and on photosynthesis was investigated. DEAE-dextran induces an increase o f the permeabi...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1977
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.59.1.15